Optimisation of reverse transcriptase loop-mediated isothermal amplification assay for rapid detection of Macrobrachium rosenbergii noda virus and extra small virus in Macrobrachium rosenbergii
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منابع مشابه
A reverse transcriptase-loop mediated isothermal amplification assay (RT-LAMP) for rapid detection of bovine viral diarrhea virus 1 and 2
Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...
متن کاملMonoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus.
The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S-transferase (GST)-fusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal antibodies (MAbs). Using dot blot, Western blot,...
متن کاملTolerance of Macrobrachium rosenbergii to white spot syndrome virus
The susceptibility of Macrobrachium idella, M. lamerrae and M. rosenbergii to white spot Ž . syndrome virus WSSV was tested by immersion challenge, oral route and intramuscular injection. Their susceptibility to WSSV was compared with that of Penaeus indicus and P. monodon. The WSSV caused 43.3% and 53.3% mortality in M. lamerrae and M. idella, respectively, by immersion method and 53.3% and 66...
متن کاملReverse transcription-loop-mediated isothermal amplification assay for rapid detection of hepatitis E virus.
The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a d...
متن کاملParvo-like virus in the hepatopancreas of freshwater prawns Macrobrachium rosenbergii cultivated in Thailand.
A survey of cultivated giant freshwater prawns Macrobrachium rosenbergii from Thailand revealed the presence of unusual spherical to ovoid inclusions in nuclei of hepatopancreas tubule epithelial cells. These began as small eosinophilic inclusions that became more basophilic as they increased in size. They were present in both R-cells and E-cells but were largest and deeply basophilic only in t...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2010
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2010.03.011